LOCUS       SPBC1215     6490 bp    DNA             PLN       16-JUL-1999
DEFINITION  S.pombe chromosome II cosmid c1215.
ACCESSION   AL096846
VERSION     AL096846.1  GI:5531459
KEYWORDS    actin cytoskeleton organisation; COX complex biogenesis; DEC1
            homologue; mitochondrial inheritance; SURF-family protein.
SOURCE      fission yeast.
  ORGANISM  Schizosaccharomyces pombe
            Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes;
            Schizosaccharomycetales; Schizosaccharomycetaceae;
            Schizosaccharomyces.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Lyne,M.H., Rajandream,M.A., Barrell,B.G., Seeger,K., Quail,M. and
            Harris,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUL-1999) European Schizosaccharomyces genome
            sequencing project, Sanger Centre, The Wellcome Trust Genome
            Campus, Hinxton, Cambridge CB10 1SA, E-mail: barrell@sanger.ac.uk
            and
COMMENT     Notes:
            Details of yeast sequencing at the Sanger Centre are available on
            the World Wide Web.
            (URL, http://www.sanger.ac.uk/Projects/S_pombe/)
            During 1995 to 1996 about 66% of S. pombe chromosome 1 was
            sequenced by the Sanger Centre.  The sequencing of the S. pombe
            genome is now being continued with funding from The European
            Commission. Fourteen European sequencing laboratories, including
            the Sanger Centre, are participating in the project.
            Protein coding regions (CDS) have been predicted with the help of
            computer analysis using the Genefinder program in PomBase (an ACEDB
            database) with additional predictions for the branch-acceptor sites
            supplied by the program Sp3splice. CAUTION: It is possible that for
            any individual CDS we may have underestimated or overestimated the
            number of introns/exons or we may not have chosen the correct
            splice donor/acceptor sites. 
            CDS are numbered using the following system eg SPBC25H2.01c. SP (S.
            pombe), B (chromosome 2), c25H2 (cosmid name), .01 (first CDS), c
            (complementary strand).
            The more significant matches with motifs in the PROSITE database
            are also included but some of these may be fortuitous. 
            The length in codons is given for each CDS.
            IMPORTANT: This sequence MAY NOT be the entire insert of the
            sequenced clone.  It may be shorter because we only sequence
            overlapping sections once, or longer, because we arrange for a
            small overlap between neighbouring submissions. 
            Cosmid c1215 is overlapped at the 5' end by cosmid c1750, EMBL
            entry SPAB4534, accession number AB004534, and at the 3' end by
            cosmid c83, EMBL entry SPBC83, accession number AL035536.
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Schizosaccharomyces pombe"
                     /strain="972h-"
                     /db_xref="taxon:4896"
                     /chromosome="II"
                     /map="IIL"
                     /clone="cosmid c1215"
     misc_feature    complement(1..82)
                     /note="nominal overlap with cosmid SPAB4534 (c1750) S.
                     pombe chromosome 2"
     tRNA            36..107
                     /note="tRNA Thr anticodon TGT, Cove score 78.51"
                     /product="tRNA-Thr"
     rRNA            415..581
                     /note="SPRG5SD K00771 Yeast (s.pombe) 5s rrna gene and
                     flanks"
     CDS             join(1501..1760,1844..2456)
                     /gene="SPBC1215.01"
                     /note="SPBC1215.01, len:290, SIMILARITY:Saccharomyces
                     cerevisiae, YG2X_YEAST, hypothetical 45.1 kd protein in
                     clb6-spt6 intergenic region, (389 aa), fasta scores: opt:
                     385, E():2.8e-19, (29.0% identity in 314 aa)"
                     /codon_start=1
                     /label=SPBC1215.01
                     /product="putative SURF-family protein"
                     /protein_id="CAB50922.1"
                     /db_xref="GI:5531460"
                     /translation="MFWWKSATKFTFSKRGPCVFRYLSTLEGTTVRPKKNKFLVGLLS
                     AVPIVTFALGTWQVKRREWKMGIINTLTERLQQPAILLPKTVTEQDTKKLEWTRVLLR
                     GVFCHDQEMLVGPRTKEGQPGYHVVTPFILDDGRRILVNRGWIARSFAEQSSRDPSSL
                     PKGPVVIEGLLRQHTDKPRFMMKNEPEKNSFYFLNVREFAQLKGTLPILITELQPSLT
                     PLQEADHVKRGLPLGHPLKVEIFNSHTEYIITWYSLSVVSAIMLYVYFKRGSGTSSLN
                     SAYERSKILNNKRL"
     gene            join(1501..1760,1844..2456)
                     /gene="SPBC1215.01"
     misc_feature    1761..1766
                     /gene="SPBC1215.01"
                     /note="gtacgt, splice donor sequence"
     misc_feature    1827..1843
                     /gene="SPBC1215.01"
                     /note="ctaacataatcacttag, splice branch and acceptor"
     CDS             complement(join(2495..2602,2663..2881,2953..4071,4111..4372,4413..4849,4889..5007,5251..5389,5434..5466))
                     /gene="SPBC1215.02c"
                     /note="SPBC1215.02c, len:811, SIMILARITY:Saccharomyces
                     cerevisiae, DEC1_YEAST, dec1 protein, (796 aa), fasta
                     scores: opt: 184, E():0.00014, (23.4% identity in 577 aa)"
                     /codon_start=1
                     /label=SPBC1215.02c
                     /product="similar to yeast DEC1 mitochondrial inheritance
                     and actin cytoskeleton organisation protein"
                     /protein_id="CAB50923.1"
                     /db_xref="GI:5531461"
                     /translation="MRRSGSKESTIVYSALSLAQAGRGPEALALLEPLKSTPINSLEL
                     LDIIQAVYDDQKKGEESFVFWEKFLQTYGKQEKNLLAYFKASIRIKSLSHQRKAAVEL
                     QKNFPSRKHTLWVISSLYLLSKKSENEVEQRLLKALAEKTAKLIFEKPTGYIDSCEEF
                     HLYLDVLLLVGDKDRALDALIHQDADRFVDADADLLLRKLELLASCARWDSLFTFSLS
                     LFQTGNTDWKVCKALLDSASNDDSKLVPLKDCILKALSTSSTKRNLHLLWIEASARFF
                     PEEHESALLGYIKKLYMKPIVFEDLRPYLLKLNVDAQHRLLDAFKLADLGESNESQKV
                     DKLYAEVLLLKIHFLLFESFTAESVVDYVRRCFVAFEKGLSLSKGLLPTDFTHGYEAL
                     LLAVHSLIYMWEGNKDLKPAEKQALIFDAICLLEKGITYSQHNFHLKLPLIRLYLLLD
                     GGFPAAAKVYDTMSIKQIQNDTLDHYLLTRATTYYPSSVTSHYINSSLKIYGSNEFET
                     PEMISMAYEDGAYSQIEDMRNFRSRLDHSTWKSISLVERARIHYLTAFKPPKQYLPKC
                     SSPKDNRDLKVFADYGSDKLPTVEESLRNSPKPDTLWIHLTVIGHSLVQDSIVNGDFE
                     KAVLSAKEMEVLCENNDLSKQLTSEEIVHMKLLIQLGLLSVKVKNGDYENSSFETIEN
                     LIESFDYENSTPLSQLTKYTEIINDLITCLNSFLYHVSATKKKEFTRQYQLLKNISSN
                     KLGSISGITKHKKKAARKYVSELLSNSWLSNLSETQVPYDPKFAKQVGEGMIDSYIQT
                     TDAVSKLPKFVKF"
     gene            complement(join(2495..2602,2663..2881,2953..4071,4111..4372,4413..4849,4889..5007,5251..5389,5434..5466))
                     /gene="SPBC1215.02c"
     misc_feature    complement(2603..2612)
                     /gene="SPBC1215.02c"
                     /note="ctaataatag, splice branch and acceptor"
     misc_feature    complement(2657..2662)
                     /gene="SPBC1215.02c"
                     /note="gtacgt, splice donor sequence"
     misc_feature    complement(2882..2896)
                     /gene="SPBC1215.02c"
                     /note="ctaaccgaattatag, splice branch and acceptor"
     misc_feature    complement(2947..2952)
                     /gene="SPBC1215.02c"
                     /note="gtaagt, splice donor sequence"
     misc_feature    complement(4072..4085)
                     /gene="SPBC1215.02c"
                     /note="ttaactcgtcgtag, splice branch and acceptor"
     misc_feature    complement(4105..4110)
                     /gene="SPBC1215.02c"
                     /note="gtaagt, splice donor sequence"
     misc_feature    complement(4373..4387)
                     /gene="SPBC1215.02c"
                     /note="ctaacttctccatag, splice branch and acceptor"
     misc_feature    complement(4407..4412)
                     /gene="SPBC1215.02c"
                     /note="gtaagt, splice donor sequence"
     misc_feature    complement(4850..4861)
                     /gene="SPBC1215.02c"
                     /note="ctaactcctcag, splice branch and acceptor"
     misc_feature    complement(4883..4888)
                     /gene="SPBC1215.02c"
                     /note="gtaaga, splice donor sequence"
     misc_feature    complement(5008..5021)
                     /gene="SPBC1215.02c"
                     /note="ttaactatttgaag, splice branch and acceptor"
     misc_feature    complement(5245..5250)
                     /gene="SPBC1215.02c"
                     /note="gtaagt, splice donor sequence"
     misc_feature    complement(5390..5405)
                     /gene="SPBC1215.02c"
                     /note="ttaacatcctttttag, splice branch and acceptor"
     misc_feature    complement(5428..5433)
                     /gene="SPBC1215.02c"
                     /note="gtaagt, splice donor sequence"
     misc_feature    6387..6490
                     /note="nominal overlap with cosmid SPBC83 S. pombe
                     chromosome 2"
BASE COUNT        0 a      0 c      0 g      0 t
ORIGIN
        1 tatatataat ttaataaata cattccgacg atactgcctc tatggcttag tggtacagca
       61 tcgcacttgt aatgcgaaga tccttggttc gattccgagt ggaggcatat acattatatt
      121 atattctttt tcatgcggaa aaaagatttc aaatttttgg gtatgatatt aatatgactg
      181 taacgttaat agcaaagtga gtgttaataa tgataaaata gcagcaaaat ctcttttccg
      241 agtaagacgt tttccagtct aaatttggag tctgcagttg tttcgcaatt cttaatgtat
      301 ggttatacta aatacaaact ttaaagctct gatttatgtt tgcaataaac taaaataaaa
      361 gcacaaaaac ctttacccat taatttcaaa caacttataa actaccggta aacttttttt
      421 ctaaccttta taatttataa actagaatgt ttaatgtcta cggccatacc taggcgaaaa
      481 caccagttcc cgtccgatca ctgcagttaa gcgtctgagg gcctcgttag tactatggtt
      541 ggagacaaca tgggaatccg gggtgctgta ggctattttt ttatatccgt ctttcttact
      601 acttgcctaa caagtcatga tgtactctca aaatatgttt gcatgccttg taatattggt
      661 tatggatagc tccttctgga cttgatcttt tgtagccaag aacaatgggt atagactctg
      721 accttgtgat gttgtagcca cagattataa taggtatttt caagtacagt aacaaaaatc
      781 ttctagtttt tttttagaaa ggatacacca agtataagca aattcaggaa ttgttgatta
      841 aactgtcaac ttcggtaaaa ctttgggcat aagtagtgtg ggagcaagtt taactaaaat
      901 tctattcaga tgtcgaatcc aaaccgctaa ttttgctcaa ctagcttttc ataaaaacca
      961 attcatagtt tcatactaat aaagacgatt gtttacttta aaacatacgt cgtaagaaca
     1021 tatattgctt tatcgaaaga taacaaatgt taagctatta tattatttaa ctatagcgca
     1081 gatttcgctt cctttactta aaaaagacat gtgacttgta gaagcttgga gtgaatacgc
     1141 aaaggtacct acttagacat tcgcgtctct cttagctgtc aacatcaaca aactggcccc
     1201 gtattgaaca gtatcttact tgtcgaagga tttgactaag aaaattttat ttctttatag
     1261 caatattccg ttttcgctta gaagattcta gtcaattgcc ctattctact tacgctttac
     1321 agtagtatca gaagacctga gtgggatttt gctgctagta gaggccattc aagttaactc
     1381 cgttttgcaa cattttaaaa gtttttgaat tgaatataaa tatcaattgt ttgattcctt
     1441 ttaggattta atcttttctt tttatttttg tttcgattga atcttggatt cctgtctatc
     1501 atgttttggt ggaaaagtgc tactaaattc acattctcaa agcgtggacc gtgtgtcttt
     1561 cgctatttga gtactcttga aggaacaact gtgaggccta aaaaaaataa atttttagtt
     1621 ggattgcttt ctgccgttcc aattgtcacg tttgctttag gaacttggca ggtaaagcga
     1681 cgagaatgga aaatgggtat catcaataca ctcacggaaa ggcttcaaca gcccgcaatt
     1741 ttattaccga aaactgttac gtacgttaag ttaacatata cacaaattgc acgttttgca
     1801 attgaactgt cgttttttac attaagctaa cataatcact tagagagcaa gatacaaaaa
     1861 aacttgagtg gactagggtt ttgcttcgtg gtgtgttttg tcacgaccaa gaaatgttgg
     1921 tcggtccaag aacgaaggaa ggccaacctg gctatcacgt agtaacccca tttattttag
     1981 acgatgggcg tcgaatttta gtcaacagag gatggattgc tcgatcattt gctgaacagt
     2041 cttctcgaga tcctagttct ttacctaaag gtccagtggt cattgaaggt cttttgagac
     2101 aacatactga taagccaaga tttatgatga agaatgagcc tgaaaaaaat tctttttact
     2161 tcttaaatgt tcgtgagttt gcacaattga aaggaactct ccccattttg ataacagaac
     2221 tacaaccatc gcttacaccg ttgcaagaag ccgatcatgt taagagaggc ttgcctcttg
     2281 gtcatcctct aaaagttgaa attttcaaca gtcatacaga atatattatc acttggtatt
     2341 ctctaagtgt ggtatcagct ataatgcttt acgtctattt taagagaggt tcaggcacat
     2401 cttctctgaa ttctgcatac gaaagaagca agattctaaa caacaaacga ttataaaaaa
     2461 ttttcatatt tataagtttc taaatattat ctacctaaaa ttttacaaat tttggaagct
     2521 tgcttactgc gtccgtcgtt tgaatgtatg aatcgatcat tccttcacca acttgttttg
     2581 caaacttcgg atcgtaagga acctattatt aggaagttag ttctcatgcc tataatttta
     2641 atgctctata atcatcacgt acctgagttt cagataaatt gctcagccaa gagtttgaca
     2701 acaattctga tacatatttt ctggcggcct tctttttatg cttcgtaatt ccagagatac
     2761 ttcctaattt gttactgctg atgtttttaa gaagttggta ttgtctggta aattcttttt
     2821 tcttggtagc tgacacgtga tataggaagc tgtttagaca agtgataagg tcatttatga
     2881 tctataattc ggttagttta gaaattttta tatcattttt tttaaaaaaa aaaaccagtt
     2941 tggtaaactt acttcagtgt actttgtcaa ttgacttaaa ggcgtggagt tctcataatc
     3001 gaatgactca attaaatttt caatcgtttc gaacgaggaa ttttcgtagt ctccgttctt
     3061 gacctttacc gaaagtaatc ccagttggat caataatttc atatgaacaa tttcttccga
     3121 agttaattgc ttcgataaat cattgttttc gcacaaaact tccatttcct tagcacttaa
     3181 aacggccttt tcaaaatcgc cgtttactat gctgtcttga acgagagaat gaccaatgac
     3241 agtcaaatgg atccaaagcg tgtccggttt aggcgaattt cttaaacttt cttcaactgt
     3301 tggcaattta tcagatccat aatctgcaaa gacctttaaa tctcgattat ctttcggaga
     3361 tgaacatttg ggaagatact gtttgggtgg tttaaaagcg gtaagataat gtatcctagc
     3421 ccgctcgacc aaagaaatgc ttttccaggt actgtggtct aaacgggatc ggaaattacg
     3481 catatcttcg atttgagagt aagcaccatc ttcataagcc atagaaatca tctctggtgt
     3541 ttcaaactca ttcgaaccat atattttgag acttgagtta atatagtgag aagtaacaga
     3601 agatggataa taggtagtag cacgagtcaa gagataatga tcaagggtat cgttttgaat
     3661 ttgcttaatg gacatagtgt cgtaaacttt agcagcagca gggaagcctc catcaagaag
     3721 aagatataaa cgaataaggg gtagctttaa atgaaagtta tgttgactat aagtaatgcc
     3781 tttttctaaa agacagattg cgtcaaaaat taaagcttgt ttttcggcag gttttaaatc
     3841 cttattcccc tcccacatat atatcaatga atgaactgct aataacaaag cttcataacc
     3901 gtgcgtaaag tcagtgggta ataagccttt acttaaagac aaaccctttt caaatgcgac
     3961 gaagcatcta cgcacgtaat cgacaaccga ttctgcagta aacgactcaa agagcaaaaa
     4021 atggatcttc agaagcaaaa cctcagcata taatttatcg actttttgag actacgacga
     4081 gttaatattg aaagatgaat aaatacttac ttcattggat tcacctagat cagccaactt
     4141 aaatgcatct aataaccggt gttgtgcatc aacattcagc ttcaatagat aaggtctcaa
     4201 atcttcaaaa acaattggtt tcatatacag ctttttgata tagccaagta atgctgattc
     4261 gtgttcttca ggaaaaaacc gtgcgctggc ttcaatccaa agtaaatgaa gatttctttt
     4321 tgtgctgctg gttgacaacg ctttcaatat acaatccttt aatgggacta atctatggag
     4381 aagttagtaa taatgcaaaa aaaaaaactt actttgaatc atcattggaa gcactatcca
     4441 gcaaagcttt gcaaaccttc caatcagtat tcccagtttg gaaaagagaa agcgaaaacg
     4501 tgaataaaga atcccaacgc gcacaggaag caagcaattc aagcttcctc aaaagaaggt
     4561 cggcgtcagc atcaacaaag cgatcagcat cttgatgtat taaagcatct aacgctctgt
     4621 ccttgtctcc cactaaaagc aacacgtcta aataaagatg aaactcctca caagaatcga
     4681 tataacctgt tggtttttcg aaaatgagtt tagcagtttt ttcagctagt gctttcagca
     4741 aacgttgttc aacctcattt tccgactttt tggacaaaag atacaaagat gagataaccc
     4801 ataaggtatg tttcctagaa ggaaagttct tttgcaattc tacagcagcc tgaggagtta
     4861 gtaaaaaaaa aacaaattaa gatcttacct ttctttggtg agacaatgat ttgatgcgaa
     4921 tagatgcttt gaagtaagct aataagtttt tttcctgctt tccataagtt tgcaagaact
     4981 tttcccaaaa tacaaacgat tcttctcctt caaatagtta ataacttaaa agtaagaggg
     5041 taaaaacaag tccattgaga aataaaacgt tgcacttgaa tcatcaagct acgaaaagaa
     5101 caaagatcgt ttttcttaag caaagtttaa ccgatcaaat aactaaagcc tttatctcaa
     5161 ggaaatagta gaatattcaa aataaaaaat acgaccaaag tgctatcaac atttatgttc
     5221 atctgttcaa tagaacctaa ttatacttac ccttcttttg atcatcataa acagcttgta
     5281 taatgtctaa aagttccaag ctgttgatgg gagtcgattt taacggttct aaaagtgcta
     5341 gagcctcggg accacgtcct gcctgagcca aggataacgc tgaataaacc taaaaaggat
     5401 gttaacagct aaaaacataa ctcgattact tacaattgtt gattctttac tcccagaacg
     5461 acgcattgta gtaggcaatg aaattaaagc ccaaacagta gtcagaagtt ggtaggtgtt
     5521 gcagtcaaat tattattcta cagaggagaa tattatagcc agcgtggtag aatctggata
     5581 tatatctact gcaaaagtgt aattgcattg gtttaaaggg tatactatgg ttaagtaata
     5641 tattcacagc tgtacaattt acagtcataa ctaaaacttc cttaagccgt aaagaaatac
     5701 ctggtgttgt aaaatttgtt gtatatccac ggcatggtca tataatgtga ttttgtgctc
     5761 aaataaatat aaaatatgca taatttttgt acatttaatt tgagaaaccc atcttttgtt
     5821 gagaggctgt caatgaatag cagtttcatt gaaaagcagc gggatgacca gaaaagtatt
     5881 ttacaatggc aagggagtag aaagctagcg taatattcag aaagctaggt aattgagcaa
     5941 tcctttaatt cattgctaag catgctaggt aaacgcagta aacctttcag ttttcattta
     6001 ggtataaggc tgtttaatga gtatctccac taaatttaaa gatcaaaact cagtatcaat
     6061 tcttaaaagt tttattttat ttaataatca tatacttctc ataatctttc aattttttcc
     6121 ccattttgat gatattttta ttaatcctac agtaagctct atgatatcgt tattcttcaa
     6181 ataggctggt cagcacgtgg acggtgttac ttatcgttaa ataaatcgta ctaaggaggt
     6241 gcgatgtaaa tgatatgctt gtcaagtatt aactgctctc caccaaccgc cggtttaact
     6301 gattattgtt gaaaagcgca gacgaagttt agagaattac tagcgtattt taaatttaat
     6361 caacggacta ttttttattc ctttgagatc cgactttatc gctttgcttc taattttcca
     6421 aaattcagtc tatctacgcg atccagccct gtttgcgtaa atttcatatt atttttcttt
     6481 aaacgtttgg
//
